Research in etiology of MONW based on epigenetics and constitution Identification of traditional Chinese and medicine theory: design and protocol
نویسندگان
چکیده
Background: We aimed in this study to evaluate the constitution status of Traditional Chinese Medicine (TCM) in metabolic obesity normal weight (MONW) and the genetic effectors of this phenotype from single nucleotide polymorphisms (SNP) and DNA methylation modification. Methods: One to one matched case-control study was conducted in our research. We conducted the standardized Constitution in Chinese Medicine Questionnaires (CCMQ) questionnaire, testing anthropometric measurements, genotyping the six gene polymorphism loci (including FTO rs1421085, rs9939609, rs1121980, PPP1R3B rs2126259, rs9987269 and GCKR rs780094) by the TaqMan probe, and using pyrosequencing to detect DNA methylation level of FTO, PPP1R3B and GCKR gene. Discussion: The results of this research will provide convinced evidence for etiology of MONW from the constitution status of TCM, genetic effects, and interactions among them and behavior risk factors laying the foundations of individualized prevention and the Precision Medicine.
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